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. 2019 Nov 8;12:249. doi: 10.3389/fnmol.2019.00249

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Copyright © 2019 Akula, Zhang, Viotti, Nestvogel, Rhee, Ebrecht, Reim, Wouters, Liepold, Jahn, Bogeski and Dresbach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Targeting and self-interaction properties of phospho-deficient mutants of Mover. (A) The T13A mutant and the T64A mutant of Mover co-immunoprecipitates with Mover-myc in transfected HEK293 cells, indicating that they undergo homomeric interaction. The data represent two independent experiments. (B,C) The two phospho-deficient mutants are targeted to presynaptic sites in rat cultured hippocampal neurons. This is indicated both by the punctate staining pattern (inverted gray level images) and the colocalization with the synapse marker Synaptophysin (small panels, representing zoomed version of the boxes). The distribution of each construct was analyzed in more than 15 neurons from three independent experiments.