Figure 1.
A. hydrophila-induced increased mortality in copper-stressed larvae. (A) A. hydrophila used in this study induced increased expression of immune-related genes in zebrafish larvae at 6 hpi (hours post infection), with increased expression observed in il-6, TLR signaling genes, MAPK signaling genes, stat1a, c3a, defb1, and hsp70.3 after only immersion infection with A. hydrophila. (B) Larvae were immersed with a different dosage of A. hydrophila at 68 hpf (hours post fertilization), and cumulative mortalities of the no-copper-stressed control and the copper-stressed larvae were calculated separately at 6, 12, 24, 36, 48, 60, and 72 hpi. (C) Larvae were immersed only or immersed after injury with 107 A. hydrophila at 68 hpf, and cumulative mortalities of the no-copper-stressed control and copper-stressed larvae were calculated separately at 6, 12, 24, 36, 48, 60, and 72 hpi. Three biological replicates were performed. ANOVA post-hoc Tukey's test. Data are presented as mean ± SD. ***P < 0.001; **P < 0.01; *P < 0.05.