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. 2019 Nov 8;10:2599. doi: 10.3389/fimmu.2019.02599

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Copyright © 2019 Chen, Luo, Xu, Chang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased apoptosis of macrophages and neutrophils in copper-stressed larvae. Annexin-V [(A2–A10), in red boxes] and PI (B1–B4) labeling of apoptotic macrophages and neutrophils, and GFP-positive cells were selected for the analysis [(A1), in blue box]. FACS panels (A2,A3) and percentage of apoptotic macrophages and neutrophils (A4) in copper-stressed coro1a promotor-driven GFP transgenic larvae; FACS panels (A5,A6,A8,A9) and percentages of apoptotic neutrophils in copper-stressed mpx transgenic larvae (A7) or lyz transgenic larvae (A10). Three biological replicates were performed. ANOVA post-hoc Tukey's test. Data are presented as mean ± SD. ***P < 0.001; and ns, no significance.