Extended Data Figure 6 |. Uptake activities of interface mutants and characterizations of all NKCC1 mutants in this study.

a, Uptake activities of NKCC1 mutants at the TM and the cytosolic domain interface. ⁸⁶Rb⁺ uptake of NKCC1 mutant was normalized to that of WT (mean± s.e.m., n=4 independent experiments except for WT, n=7 independent experiments and for WT with bumetanide, n=3 independent experiments). b, NKCC1 WT and mutants (also including those in Fig. 1e, 3e, 4f) in size exclusion chromatography. The GFP-fusion protein is monitored by fluorescence. Experiments were repeated 3 times independently with similar results. c, The expression level of NKCC1 WT and mutants as shown by Western blot. Experiments were repeated 3 times independently with similar results. For gel source data, see Supplementary Figure 2. d, The membrane localization of NKCC1 WT and mutants. The fluorescence images are shown for HEK293 cell expressing NKCC1-GFP fusion. Experiments were repeated 3 times independently with similar results.