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. 2019 Nov 14;9:16746. doi: 10.1038/s41598-019-53196-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Volcano plot and MS/MS spectra of up-regulated proteins expressed in 3D co-cultured adipocytes with macrophages (3C) versus 2D mono-cultured preadipocytes (2P). (A) Volcano plot of quantified proteins between 3C and 2P illustrates the log₂ 3C/2P iTRAQ ratio (x-axis) and –log p-value (y-axis). The non-axial vertical lines indicate ±1.5-fold change, and the non-axial horizontal line indicates p = 0.05, which is the statistical threshold for measuring differentially expressed proteins. The 48 proteins denoted in red among 124 up-regulated proteins were included in the cluster. (B) MS/MS spectra of MDH1 (FVEGLPINDFSR), FABP5 (ELGVGLALR) and FH (VLLPGLQK), which are involved in glycolysis/gluconeogenesis, TCA cycle, and fatty acid metabolism. Left spectra indicate the iTRAQ reporter ions. 2P, 114.11; 2D mono-cultured adipocyte (2A), 115.11; 2D co-cultured adipocytes with macrophages (2C), 116.11; 3D mono-cultured preadipocytes (3P), 117.11; 3D mono-cultured adipocyte (3A), 118.11; 3C, 119.11.