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. 2019 Nov 14;9:16743. doi: 10.1038/s41598-019-52466-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EC^iZSGreen mice secrete ZSGreen⁺ EC-EVs into plasma. (a) FACS plots using EVs isolated from wildtype (ZSGreen⁻) or EC^iZSGreen mice. (b) ZetaView NTA plots of bright field and fluorescence using EVs isolated from the plasma of EC^iZSGreen mice. (c) Still images from ZetaView NTA movies using EVs isolated from EC^iZSGreen mice. (d) Quantification of total and fluorescent ZSGreen⁺ EVs from control versus mammary tumor bearing mice using ZetaView NTA (EVs were pooled from n = 3 mice; 3–7 biological replicates). Results were analyzed using Student’s t-test, *p < 0.05.