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. 2019 Nov 14;9:16854. doi: 10.1038/s41598-019-53319-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optical microscopy images of ECM mineralization (Von Kossa staining) within the core of the 3D CHT/G scaffold under either static (a) or dynamic (perfusion and compression) (c). Corresponding maximum entropy threshold-based image segmentation is also shown (b,d). Quantitative analysis of the total amount of scaffold area covered by the von Kossa black spots is shown (e). *Statistically significant difference (Student’s t-test; p < 0.05). Scale bar = 250 μm.