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. 2019 Nov 8;10:1221. doi: 10.3389/fpls.2019.01221

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Copyright © 2019 Wu, Kang, Wang, Haider, Price, Hajek and Hanzawa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of photoperiod, time point, and genotype on expression of the non-flowering genes and flowering genes. The average numbers of variable conditions in which a gene showed significant differential expression when one variable is fixed (photoperiod, time point, or genotype) for the non-flowering genes (black columns) or the flowering genes (gray columns). For instance, under long-day photoperiod, there are possible variable conditions of 18 (6 genotypes × 3 sampling time points). Since there are three photoperiod regimes, the total number of conditions is 54 under fixed photoperiod. Similarly, the total number of conditions under fixed time point or under fixed genotype is 54. Whitney nonparametric test was employed to examine the statistical significance difference ( p > 0.05) in differential expression using Prism v.6.0.