Table 1.
pH and SCFA mean ± SEM reported in µmol/gram wet weight faeces.
| Fresh Frozen | Collection tubes | P-value | |
|---|---|---|---|
| pH | 7.31 ± 0.06 | 7.42 ± 0.06 | 0.009* |
| Propionic acid | 20.24 ± 2.16 | 19.22 ± 1.86 | 0.483 |
| Isobutyric acid | 2.47 ± 0.23 | 2.33 ± 0.22 | 0.434 |
| Butyric acid | 18.36 ± 1.83 | 17.58 ± 2.58 | 0.136 |
| Isovaleric acid | 3.84 ± 0.39 | 3.61 ± 0.40 | 0.561 |
| Valeric acid | 2.53 ± 0.14 | 2.65 ± 0.24 | 0.896 |
| Caproic acid | 1.25 ± 0.18 | 1.90 ± 0.47 | 0.288 |
| Acetic acid | 62.14 ± 5.37 | 54.33 ± 7.70 | 0.002* |
| Acetic acid – non-compliant (>2 grams faeces) (n = 21) | 65.76 ± 6.12 | 57.45 ± 9.30 | 0.011* |
| Acetic acid – compliant (≤2 gram of faeces) (n = 5) | 46.94 ± 9.00 | 41.23 ± 7.5 | 0.063 |
Significant differences between methods were determined with the Wilcoxon Signed Rank test after False Discovery Rate (FDR) correction (*P < 0.050).