Table 2.
Relative microbial abundance (%) mean values ± SEM at phylum-level.
| Phylum-level: fresh frozen vs tube collections | P-value | ||
|---|---|---|---|
| Fresh frozen | Collection tubes | ||
| Actinobacteria | 11.42 ± 1.36 | 5.78 ± 0.81 | 0.001* |
| Bacteroidetes | 7.7 ± 0.93 | 32.27 ± 1.56 | <0.001* |
| Euryarchaeota | 2.05 ± 0.52 | 0.7 ± 0.15 | 0.900 |
| Firmicutes | 77.63 ± 1.34 | 58.5 ± 1.43 | <0.001* |
| Lentisphaerae | 0 ± 0 | 0.1 ± 0.03 | <0.001* |
| Proteobacteria | 0.24 ± 0.04 | 1.46 ± 0.14 | <0.001* |
| Synergistetes | 0 ± 0 | 0.05 ± 0.03 | 0.860 |
| Verrucomicrobia | 0.91 ± 0.19 | 1.06 ± 0.21 | 1.000 |
| Phylum-level: compliant (≤2 grams) (n = 21) vs non-compliant (>2 grams)(n = 5) | |||
| Non-compliant | Compliant | P-value | |
| Actinobacteria | 5.90 ± 0.90 | 5.10 ± 1.91 | 1.000 |
| Bacteroidetes | 31.07 ± 1.57 | 39.48 ± 5.00 | 0.260 |
| Euryarchaeota | 0.79 ± 0.17 | 0.13 ± 0.13 | 0.040* |
| Firmicutes | 59.67 ± 1.44 | 51.47 ± 4.46 | 0.220 |
| Lentisphaerae | 0.06 ± 0.01 | 0.36 ± 0.15 | 0.120 |
| Proteobacteria | 1.51 ± 0.16 | 1.13 ± 0.22 | 0.880 |
| Synergistetes | 0.05 ± 0.03 | 0.01 ± 0.00 | 1.000 |
| Verrucomicrobia | 0.87 ± 0.20 | 2.21 ± 0.78 | 0.160 |
Data generated from MiSeq. 16S rRNA gene sequencing. Significant differences were determined with the Wilcoxon Signed Rank test after False Discovery Rate (FDR) correction (*P < 0.050 is considered significantly different).