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. 2019 Nov 14;9:16802. doi: 10.1038/s41598-019-53191-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Upregulations of TIPRL, LC3 and CD133 in HCCs. Human tissues were stained with the indicated antibodies followed by confocal observation. (a) The levels of TIPRL, LC3 and CD133 were determined using the ZEN program (Supplementary Tables 1 and 2). p-values (^*P < 0.05; ^**P < 0.01) were determined by a paired t-test, and % differences are shown. (n) is the number of samples. (b) The representative images were selected from normal and HCCs groups, respectively. DAPI was used for nucleus staining, and scar bars, 20 (inserted) and 100 μm.