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. 2019 Nov 14;9:16791. doi: 10.1038/s41598-019-53069-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The CysA2 protein can bind to pulmonary mouse cells. The protein-TC-1 cells binding assays were performed with purified CysA2 protein. After blocking Fcγ receptors, cells were treated with CysA2 and further stained with purified anti-His-tag IgG monoclonal antibody. The IgG molecule was detected by the anti-IgG-PE. (a) Flow cytometry data analysis by histograms, which measure and compare only a single parameter on the X-axis, being the amount of anti-his antibody fluorescence from the control group (only TC-1 cells) versus the CysA2 group (TC-1 cells treated with CysA2). (b) Representative graph of the median of fluorescence intensity comparing the control group versus the CysA2 group. The analysis was performed in LSR Fortessa flow cytometers (BD Bioscience), and data were analyzed by FlowJo software (version 10.0.7 - Tree Star). Experiments were performed twice, in triplicate. MFI - median of fluorescence intensity; ***p < 0.001.