Table 1.
Kinetic parameters obtained for M. tuberculosis CysA2 in comparison with TSTs and MSTs previously characterized in the literature.
| Enzymes TST |
Enzyme activity | Reference | |||
|---|---|---|---|---|---|
| Km cyanide (mM) |
Km thiosulfate (mM) |
Km 3-mp (mM) |
Vmax (µmol.min−1.mg−1) |
||
|
Mycobacterium tuberculosis CysA2 (Mtu0100) |
1.66 | 2.97 | — | 0,008 × 10−3 | This work |
|
Arabdopsis thaliana (ATIG16460) |
— | 7.2 | 5.4 | — | 58 |
|
Rattus norvergicus (Rno25274) |
— | 4.4 | 2.6 | 1.1 × 104 | 59 |
|
Bos taurus Rhobov (Bta280946) |
0.063 | 7.0 | — | 600 | 12 |
|
Azotobacter vinelandii RhdA (AvCA607450) |
8.7 | 1.0 | — | 1250 | 30 |
|
Pseudomonas aeruginosa RhdA (PaePA4956) |
16 | 7.4 | — | 815 | 16 |
|
Fusarium solani (Necha2_57025) |
2 | — | — | 0.083 | 17 |
|
Trichoderma harzianum (COG2897) |
7 | — | — | 0.069 | 17 |
|
Mycobacterium tuberculosis CysA2 (Mtu01000) |
9.11 | — | 7.02 | 0,0461 × 10−3 | This work |
|
Escherichia coli SseA (Ecob2521) |
1.25 | — | 8.34 | — | 60 |
|
Homo sapiens (Hsa4357) |
130 | — | 350 | 417 | 37 |
|
Arabidopis thaliana (ATIG79230) |
— | — | 11 | 2.4 × 103 | 58 |
|
Leishmania major (LMJF050970) |
— | 10.9 | 345 | 0.2 | 32 |
|
Leishmania mexicana (LMXM050970) |
— | 20.8 | 345 | — | 32 |
|
Rattus norvegicus (Rno192172) |
— | 6.2 × 10 | 1.2 | — | 58 |
MST1_Arabidopsis thaliana (AT1G79230); MST2_Arabidopsis thaliana (AT1G16460); TST_Rattus norvegicus Rno25274); MST Rattus norvegicus (Rno192172); Rhobov_Bos Taurus (Bta280946); RhdA_Azotobacter vinelandii (AvCA607450); RhdA_Pseudomonas aeruginosa RhdA (PaePA4956); MST_Leishmania major (LMJF050970); MST_Leishmania mexicana (LMXM050970); TST_Trichoderma harzianum (COG2897); TST_Fusarium solani (Necha2_57025). (—) not identified.