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. 2019 Nov 14;9:16791. doi: 10.1038/s41598-019-53069-6

Table 1.

Kinetic parameters obtained for M. tuberculosis CysA2 in comparison with TSTs and MSTs previously characterized in the literature.

Enzymes
TST
Enzyme activity Reference
Km
cyanide
(mM)
Km
thiosulfate
(mM)
Km
3-mp
(mM)
Vmax
(µmol.min−1.mg−1)

Mycobacterium tuberculosis CysA2

(Mtu0100)

1.66 2.97 0,008 × 10−3 This work

Arabdopsis thaliana

(ATIG16460)

7.2 5.4 58

Rattus norvergicus

(Rno25274)

4.4 2.6 1.1 × 104 59

Bos taurus Rhobov

(Bta280946)

0.063 7.0 600 12

Azotobacter vinelandii

RhdA

(AvCA607450)

8.7 1.0 1250 30

Pseudomonas aeruginosa

RhdA

(PaePA4956)

16 7.4 815 16

Fusarium solani

(Necha2_57025)

2 0.083 17

Trichoderma harzianum

(COG2897)

7 0.069 17

Mycobacterium tuberculosis CysA2

(Mtu01000)

9.11 7.02 0,0461 × 10−3 This work

Escherichia coli

SseA

(Ecob2521)

1.25 8.34 60

Homo sapiens

(Hsa4357)

130 350 417 37

Arabidopis thaliana

(ATIG79230)

11 2.4 × 103 58

Leishmania major

(LMJF050970)

10.9 345 0.2 32

Leishmania mexicana

(LMXM050970)

20.8 345 32

Rattus norvegicus

(Rno192172)

6.2 × 10 1.2 58

MST1_Arabidopsis thaliana (AT1G79230); MST2_Arabidopsis thaliana (AT1G16460); TST_Rattus norvegicus Rno25274); MST Rattus norvegicus (Rno192172); Rhobov_Bos Taurus (Bta280946); RhdA_Azotobacter vinelandii (AvCA607450); RhdA_Pseudomonas aeruginosa RhdA (PaePA4956); MST_Leishmania major (LMJF050970); MST_Leishmania mexicana (LMXM050970); TST_Trichoderma harzianum (COG2897); TST_Fusarium solani (Necha2_57025). (—) not identified.