Figure 2.

Growth in 7H9 medium and HdB minimal medium. (a) M. smegmatis WT (black) and mutant ε^4A (red) were grown in 7H9 media containing 0.2% glycerol. (b,c) Comparison of growth of M. smegmatis WT (black), ε^4A mutant strain (red) and its complemented mutant strain (blue) in HdB minimal media containing (b) 0.2% glycerol or (c) 0.2% acetate. Growth was determined by measuring cell density using optical density at 600 nm (OD₆₀₀). The growth differences of all three strains could be observed in both growing conditions, in which the ε^4A mutant strain grew slower than WT strain, and the growth was partially rescued by introducing pMV262-atpC into the mutant strain. (d) Intracellular ATP levels measured in WT, ε^4A mutant and ε^4A complemented strain at mid-log phase in minimal media containing 0.2% acetate. All growth experiments are performed in three biological replicates, and the error bar represents standard deviations.