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. 2019 Nov 14;9:16759. doi: 10.1038/s41598-019-53107-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oxygen consumption and extracellular flux analysis of mutant ε^4A. (a) Raw trace schematic of experimental setup. Respiration was initiated with 0.2% glucose and subsequently uncoupled with 10 µM CCCP at points “G” and “C”, respectively. (b) Baseline-subtracted OCR vs ECAR phenograms of M. smegmatis strains after glucose-stimulated respiration. (c) The spare respiratory capacity (SRC, calculated as [OCR_CCCP - OCR_glucose]) of M. smegmatis strains.