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. 2019 Nov 14;9:16759. doi: 10.1038/s41598-019-53107-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NMR studies of Mtε and its mutants. (a) Comparison of overlaid HSQC spectra for Mtε (blue), MtεR113A (red), and MtεR105A,R111A,R113A,R115A (green). (b) Weighted Chemical Shift Perturbations (CSPs) for the ¹⁵N and ¹H resonance between Mtε and MtεR113A; regions with residues showing CSP above the average weighted CSP value are highlighted with grey colour and labelled as one-letter code. The weighted CSPs between Mtε and MtεR113A for backbone ¹⁵N and ¹HN were calculated by the formula Δδ = [(ΔN/5)²+(ΔHN)²]^0.5. The average CSP weighted value was displayed by dashed line. (c) Ribbon representation of Mtε showing the regions and residues with significantly changed chemical shift perturbation values above average (blue).