Table 1.

Growth kinetics of the M. smegmatis WT and ε^4A mutant strain and its complemented strain in HdB minimal media supplemented with 0.2% glycerol or acetate.

	WT strain	ε4A mutant strain	ε4A complemented strain
In HdB media supplemented with 0.2% glycerol
Doubling time (h)	3.2	4.1	3.9
Dry weight at 48 h (g/l)	1.07 ± 0.02	0.98 ± 0.04	1.04 ± 0.02
Yglycerol (g/mol) at 48 h	33.07 ± 1.28	33.91 ± 2.96	37.68 ± 1.70
pH at 48 h	5.84 ± 0.04	5.95 ± 0.02	5.95 ± 0.03
OD600 at 48 h	2.26 ± 0.29	2.21 ± 0.03	2.43 ± 0.07
In HdB media supplemented with 0.2% acetate
Doubling time (h)	4.4	7.9	7.6
Dry weight at 72 h (g/l)	0.38 ± 0.01	0.24 ± 0.01	0.29 ± 0.02
Yacetate (g/mol) at 72 h	9.45 ± 0.78	5.78 ± 0.47	7.47 ± 0.43
pH at 72 h	8.30 ± 0.03	7.86 ± 0.05	8.02 ± 0.10
OD600 at 72 h	0.79 ± 0.04	0.56 ± 0.02	0.77 ± 0.04