Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 14;9:16833. doi: 10.1038/s41598-019-53208-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

AP205-SpyCatcher and SpyTag-P47 isopeptide bond formation. (A) Schematic representation of the AP205-SpyCatcher and SpyTag-P47 isopeptide bond formation. Diagrams show SpyCatcher in green, Spytag in blue, and P47 in red. (B) Coomassie blue staining of SpyTag-P47, AP205-SpyCatcher, and conjugated VLP-P47 in SDS-PAGE after boiling and reducing in SDS-loading buffer (left). Anti-his western blot of SpyTag-P47, AP205-SpyCatcher, and conjugated P47-VLP (center). Anti-Pfs47 western blot of SpyTag-P47, AP205-SpyCatcher, and conjugated VLP-P47 (right). (C) TEM of VLP-P47 after negative staining with 2% uranyl acetate. (D) Size distribution of VLP-P47 from TEM image (n = 559). The average hydrodynamic diameter is 22.48 +/− 2.26 nm. Scale bar: 50 nm.