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. 2019 Nov 14;9:16768. doi: 10.1038/s41598-019-53391-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of EPO expression in GLUL-MSX-mediated HEK293 producer cell pool. (a) Endogenous (light gray bars) and exogenous (dark gray bars) GLUL and EPO genomic DNA copy count analyzed via droplet digital PCR (ddPCR). Values represent mean ± SD (b) Endogenous (light gray bars) and exogenous (dark gray bars) GLUL and EPO mRNA copy count analyzed via ddPCR. Values represent mean ± SD (c) Immunoblot of GLUL and EPO protein in HEK293 wildtype (WT), GLUL-KO (KO #24) and cell pool #8 (CP#8). Actin was used as a loading control.