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. 2019 Nov 14;9:16789. doi: 10.1038/s41598-019-53357-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representation of the algorithm used to process the images. The images of the superficial capillary plexus (SCP) and deep vascular complex (DVC) were graded for the presence of microaneurysms (MAs – indicated with arrows) in a 1-mm square region of interest (orange square) that was temporal and tangential to the foveal center (orange star). Those MAs visualized on both plexuses were included once. The OCTA volume scans were imported in ImageJ and elaborated (please see the “Methods” section for further details) in order to obtain the rotational 3D visualization of each MA, in which OCTA-based flow information is displayed as gray. Moreover, a second 3D visualization in which structural OCT and OCTA information had different colors (gray and magenta for structural and flow information, respectively) was used to topographically correlate MAs with retinal layers.