Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 14;10:5155. doi: 10.1038/s41467-019-13033-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Clcn2^R180Q/+ mice have elevated Cyp11b2 expression and plasma aldosterone levels. a, c, d Plasma aldosterone (WT: N = 37; Clcn2^R180Q/+: N = 36; P < 0.0001; one-way ANOVA, Sidak´s MCP; F = 4.161; df = 120) and Cyp11b2 expression (WT: N = 32, 1.23 ± 0.17; Clcn2^R180Q/+: N = 25, 1.47 ± 0.1; P = 0.0431; one-way ANOVA, Sidak´s MCP of log-transformed fold change; F = 2.907; df = 63) are significantly increased in Clcn2^R180Q/+ mice. Clcn2^−/− mice have unchanged plasma aldosterone (N = 10; P = 0.999; F = 4.313; df = 118) and Cyp11b2 expression (N = 10; P = 0.9959; both one-way ANOVA including Sidak´s MCP; F = 2.907; df = 63). HSD leads to suppressed aldosterone levels in WT (WT: N = 37; HSD-WT: N = 19; P < 0.0001) and Clcn2^R180Q/+ (Clcn2^R180Q/+: N = 36; HSD-Clcn2^R180Q/+: N = 23; P < 0.0001; both one-way ANOVA, Sidak´s MCP; F = 4.313; df = 118). Cyp11b2 expression is upregulated in Clcn2^R180Q/+ compared to WT (HSD-WT: N = 24; HSD-Clcn2^R180Q/+: N = 27; P < 0.0001; Mann–Whitney test). b, e Plasma aldosterone is elevated in females (WT: N = 21; Clcn2^R180Q/+: N = 23; P = 0.0323; df = 69) and males (WT: N = 16; Clcn2^R180Q/+: N = 13; P = 0.0098; both one-way ANOVA, Bonferroni´s MCP; F = 5.589; df = 69), Cyp11b2 expression is upregulated only in male Clcn2^R180Q/+ (WT: N = 15; Clcn2^R180Q/+: N = 7; P = 0.0275; one-way ANOVA including Sidak´s MCP; F = 1.782; df = 53). f, h, i PRC is unchanged (WT: N = 37, 1474 ± 245.3 ng ml⁻¹ h⁻¹, Clcn2^R180Q/+: N = 36, 1334 ± 138.7 ng ml⁻¹ h⁻¹, P > 0.9999; HSD-WT: N = 19, 2830 ± 590.3 ng ml⁻¹ h⁻¹; HSD-Clcn2^R180Q/+: N = 23, 1522 ± 218.4 ng ml⁻¹ h⁻¹, P > 0.9999; both Kruskal–Wallis test, Dunn´s MCP). Ren1 expression is unchanged (WT: N = 32, 1.17 ± 0.12; Clcn2^R180Q/+: N = 27, 1.16 ± 0.14; P = 0.9812; one-way ANOVA including Sidak´s MCP; F = 0.7894; df = 63; HSD-WT: N = 24, 1.26 ± 0.15; HSD-Clcn2^R180Q/+: N = 27, 1.462 ± 0.23; P = 0.9813; Mann–Whitney test). In Clcn2^−/−, the expression of Ren1 (WT: N = 32, 1.17 ± 0.12; Clcn2^−/−: N = 10, 2.1 ± 0.32; P = 0.0125; one-way ANOVA including Sidak´s MCP; F = 0.7894; df = 63) and PRC (WT: N = 37, 1474 ± 245.3 ng ml⁻¹ h⁻¹; Clcn2^−/−: N = 10, 2602 ± 546.1 ng ml⁻¹ h⁻¹; P = 0.03; Kruskal–Wallis test, Dunn´s MCP) are significantly upregulated compared to WT. g, j Female (WT: N = 21, 1839 ± 392 ng ml⁻¹ h⁻¹; Clcn2^R180Q/+: N = 23, 1589 ± 166.3 ng ml⁻¹ h⁻¹; P > 0.9999;) as well as male mice (WT: N = 16, 995.1 ± 194.7 ng ml⁻¹ h⁻¹; Clcn2^R180Q/+: N = 13, 882.6 ± 198 ng ml⁻¹ h⁻¹; P > 0.9999; both Kruskal-Wallis test; Dunn´s MCP) show no significant alteration in PRC. Ren1 expression is significantly downregulated in male Clcn2^R180Q/+ mice only (WT: N = 15, 1.14 ± 0.16; Clcn2^R180Q/+: N = 7, 0.57 ± 0.07; P = 0.0158; one-way ANOVA including Sidak´s MCP; F = 0.6378; df = 53). Mean ± SEM for all data in the legend; box plots (box, interquartile range; whiskers, 1.5 times the interquartile range; line, median; dots, outliers) are shown in the figure; N values are biologically independent animals. NSD, normal salt diet. *, p < 0.05; **, p < 0.01; ****, p < 0.0001; n.s., p > 0.05