Fig. 6.
Plasma and urine electrolyte levels. Plasma levels of potassium (a) (WT: N = 10; Clcn2R180Q/+: N = 16; P = 0.9315; female WT: N = 6; female Clcn2R180Q/+: N = 10; P = 0.5490; male WT: N = 4; male Clcn2R180Q/+: N = 6; P = 0.9161; one-way ANOVA including Sidak´s MCP; F = 1.02; df = 46), sodium (b) (WT: N = 10; Clcn2R180Q/+: N = 16; P = 0.9854; female WT: N = 6; female Clcn2R180Q/+: N = 10; P = 0.9956; male WT: N = 4; male Clcn2R180Q/+: N = 6; P = 0.7652; one-way ANOVA including Sidak´s MCP; F = 0.4119; df = 46) and chloride (c) (WT: N = 10; Clcn2R180Q/+: N = 16; P = 0.5559; female WT: N = 6; female Clcn2R180Q/+: N = 10; P = 0.9658; male WT: N = 4; male Clcn2R180Q/+: N = 6; P = 0.4857; one-way ANOVA including Sidak´s MCP; F = 0.5725; df = 46) do not differ between WT and Clcn2R180Q/+ mice. Urinary potassium (d) is not changed in Clcn2R180Q/+ mice (WT: N = 13; Clcn2R180Q/+: N = 20; P = 0.7833; female WT: N = 7; female Clcn2R180Q/+: N = 10; P = 0.5567; male WT: N = 6; male Clcn2R180Q/+: N = 10; P > 0.9999; one-way ANOVA including Sidak´s MCP; F = 0.9392; df = 60). In contrast, urine levels of sodium (e) (WT: N = 13; Clcn2R180Q/+: N = 20; P = 0.0032; female WT: N = 7; female Clcn2R180Q/+: N = 10; P = 0.0030; male WT: N = 6; male Clcn2R180Q/+: N = 10; P = 0.0819; one-way ANOVA including Sidak´s MCP; F = 1.617; df = 60) and chloride (f) (WT: N = 13; Clcn2R180Q/+: N = 20; P = 0.0148; female WT: N = 7; female Clcn2R180Q/+: N = 10; P = 0.0064; male WT: N = 6; male Clcn2R180Q/+: N = 10; P = 0.7609; one-way ANOVA including Sidak´s MCP; F = 0.4131; df = 60) show significant decreases in Clcn2R180Q/+ overall and in female mice. All data are shown in box plots (box, interquartile range; whiskers, 1.5 times the interquartile range; line, median; dots, outliers); N values are biologically independent animals. *, p < 0.05; **, p < 0.01; n.s., p > 0.05