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. 2019 Nov 14;9:16881. doi: 10.1038/s41598-019-53175-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth curves for E. faecalis V583 (continuous line) and E. faecalis V583 ΔagdiΔtdc (discontinuous line) in media supplemented with 20 mM agmatine and 10 mM tyrosine. (A) influence of putrescine and tyrosine synthesis on cultures grown in GM17. (B) effect of limiting the carbon source on cells cultured in M17 supplemented with glucose at 1 g L⁻¹. (C) influence of acidic pH on the growth of strains propagated in GM17 adjusted to an initial pH of 5.0. (D) growth under a combination of carbon source depletion and acidic pH in M17 with glucose 1 g L⁻¹ and pH adjusted to 5.0. The OD₆₀₀ was monitored over 10 h.