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. 2019 Nov 14;9:16881. doi: 10.1038/s41598-019-53175-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biofilm formation by E. faecalis V583 (wt, black bars) and E. faecalis V583 ΔagdiΔtdc (ΔagdiΔtdc, white bars) on polystyrene microtitre plates in GM17 in the absence or presence of 20 mM agmatine (A) and 10 mM tyrosine (T) after 16 h. Biofilms were assessed by crystal violet staining and the data corrected for the OD₆₀₀.