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. 2019 Nov 14;10(11):867. doi: 10.1038/s41419-019-2098-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative immunoblots of Ser780 phospho-Rb, total Rb, and actin for tumor cell lysates obtained from xenografts of parental cell-line control (gray) and 48 h of palbociclib treatment (red). b Corresponding cisplatin-resistant cell-line control (black) and 48 h of palbociclib treatment (blue). c Quantification by photo densitometry was performed on bands using Image Lab Software, and the ratio of phospho-Rb/total Rb was calculated. All quantification is presented as mean ± SD. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, by Student’s t-test)