TABLE 2.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source/reference |
|---|---|---|
| D. zeae strains | ||
| MS2 | Wild type; isolated from infected banana | 1 |
| Δ5075 | C1030_05075 deletion mutant (annotated as nonribosomal peptide synthetase gene) of MS2 | This work |
| ΔexpR | expR deletion mutant of MS2 | This work |
| ΔexpI | expI deletion mutant of MS2 | This work |
| ΔpipR | pipR deletion mutant of MS2 | This work |
| ΔpipA | pipA deletion mutant of MS2 | This work |
| E. coli strains | ||
| DH5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | Invitrogen |
| C118 λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE (Am) recA1 λpir | 63 |
| SM10 λpir | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir Kanr | 56 |
| DH12S(pHV402, pLC42) | 3OC6-HSL bioassay reporter; Camr Kanr | 64 |
| Plasmids | ||
| pKNG101 | Suicide vector; strAB sacB oriR6K monRK2; Strr | 65 |
| pKNG5075 | Knockout plasmid for 5075-mutant strain construction; Strr | This work |
| pKNGexpR | Knockout plasmid for ExpR-mutant strain construction; Strr | This work |
| pKNGexpI | Knockout plasmid for ExpI-mutant strain construction; Strr | This work |
| pKNGpipR | Knockout plasmid for PipR-mutant strain construction; Strr | This work |
| pKNGpipA | Knockout plasmid for PipA-mutant strain construction; Strr | This work |
| pBBR1MCS-5 | Broad-host-range vector; Genr | 62 |
| pC5075 | pBBR1MCS-5 expressing Plac C1030_05075; Genr | This work |
| pCexpI | pBBR1MCS-5 expressing Plac expI; Genr | This work |
| pCpipA | pBBR1MCS-5 expressing Plac pipA; Genr | This work |
| pBBR1MCS-4 | Broad-host-range vector; Ampr | 62 |
| pPipAhis | C-terminal PipA-His6 expression plasmid; Ampr | This work |