TABLE 3.
Primers used in this study
| Primer | Sequence (5′–3′) | Description |
|---|---|---|
| pKNG-F | CGTATTCAGTGTCGCTGATTTGTA | Suicide vector backbone |
| pKNG-R | GGTACCATAAGTAGAAGCAGCAAC | Suicide vector backbone |
| expI-KO-UP-F | AACAACTTCAGACAATACAAATCAGCGACACTGAATACGTCACCCTGAACGGACGTGATC | Construction of ΔexpI mutant |
| expI-KO-UP-R | CGAAATGAACCTGATCAAGCTCCCCCTATCCTTAACTTCATGTC | Construction of ΔexpI mutant |
| expI-KO-DN-F | GAAGTTAAGGATAGGGGGAGCTTGATCAGGTTCATTTCGACC | Construction of ΔexpI mutant |
| expI-KO-DN-R | GGTAAAGCTACTTGGGTTGCTGCTTCTACTTATGGTACCGTGCCGTCATGATCAGATTGC | Construction of ΔexpI mutant |
| pipA-KO-UP-F | AAACAACTTCAGACAATACAAATCAGCGACACTGAATACGGATGCGGTGATTTACGACTACAC | Construction of ΔpipA mutant |
| pipA-KO-UP-R | GGTGTTAGCAACCATTAACAGGTGTACATGAGTGACGTTTACC | Construction of ΔpipA mutant |
| pipA-KO-DN-F | AAACGTCACTCATGTACACCTGTTAATGGTTGCTAACACCAGG | Construction of ΔpipA mutant |
| pipA-KO-DN-R | TGGTAAAGCTACTTGGGTTGCTGCTTCTACTTATGGTACCCAAGAGCGCATAGGTAGTCATTAG | Construction of ΔpipA mutant |
| 5075-KO-UP-F | AAACAACTTCAGACAATACAAATCAGCGACACTGAATACGGTGCTGCTGGATACCTTCG | Construction of Δ5075 mutant |
| 5075-KO-UP-R | CGCCATGCCAAAAGTTTTACCAGTCATCCACCACGTTGATC | Construction of Δ5075 mutant |
| 5075-KO-DN-F | ATCAACGTGGTGGATGACTGGTAAAACTTTTGGCATGGCGC | Construction of Δ5075 mutant |
| 5075-KO-DN-R | TGGTAAAGCTACTTGGGTTGCTGCTTCTACTTATGGTACCAAACGCGGACTGAAAGTTGG | Construction of Δ5075 mutant |
| pipA-F | CCCAAGCTTGTAACTAGCATTTTTGCTAGTG | Construction of pPipAhis |
| pipA-R | AACTGCAGAGCAACCATTAGTGATGATGATGATGATGACAATACTGCGTC | Construction of pPipAhis |
| pBBR-F | GCGCTTGGCGTAATCATGGTC | pBBR1MCS-5 backbone |
| pBBR-R | TCGTGACTGGGAAAACCCTGG | pBBR1MCS-5 backbone |
| C5075-F | AAACAGCTATGACCATGATTACGCCAAGCGCGCAAACGTTACCGCACAACCAGAG | Construction of pC5075 |
| C5075-R | TGTAGCCGCCTCGCCGCCCTATACCTTGTCTGCCTTAGCTGACTGGAGGCAATGC | Construction of pC5075 |
| CexpI-F | ACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCTCCCAGCGGGAGTTATCTCAC | Construction of pCexpI |
| CexpI-R | GATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACTAAACACGCCATTCGGCTTG | Construction of pCexpI |
| CpipA-F | CCCAAGCTTATAACTAGCATTTTTGCTAG | Construction of pCpipA |
| CpipA-R | AACTGCAGCCTACCTGGTGTTAGCAACC | Construction of pCpipA |