TABLE 5.
List of proteins whose extracellular secretion was modified in the Ck-mut strain compared to the original Ck straina
| Category | Protein accession no. |
Spectral count for strain (titer [log/ml]): |
Mnemonic | Location | Annotation | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ck (9.4) |
Ck1 (9.7) |
Ck-mut1 (9.1) |
Ck-mut5 (8.9) |
Ck-mut50 (8.7) |
||||||||||
| P | S | P | S | P | S | P | S | P | S | |||||
| Gene translation abolished | ABC01099.1 | 1 | 47 | 3 | 72 | 0 | 1 | 0 | 1 | 0 | 0 | MCAP_0843 | lpp | Transglutaminase |
| ABC01278.1 | 0 | 44 | 0 | 70 | 0 | 0 | 0 | 0 | 0 | 0 | MCAP_0860 | 1 TMB | Hypothetical protein | |
| ABC01319.1 | 0 | 25 | 0 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | MCAP_0863 | —b | DUF2570 | |
| ABC01807.1 | 0 | 100 | 11 | 146 | 0 | 0 | 0 | 0 | 0 | 0 | MCAP_0864 | 1 TMB | Topoisomerase? | |
| Gene translation and protein exosecretion reduced | ABC01143.1 | 0 | 50 | 0 | 47 | 4 | 8 | 3 | 4 | 5 | 0 | MCAP_0351 | 1 TMB | IgG-blocking virulence domain |
| ABC01224.1 | 0 | 56 | 0 | 38 | 3 | 19 | 3 | 9 | 1 | 4 | MCAP_0513 | lpp | Transglutaminase-like superfamily | |
| ABC01376.1 | 0 | 157 | 2 | 271 | 0 | 18 | 0 | 9 | 0 | 0 | MCAP_0862 | 1 TMB | DUF342 | |
| ABC01488.1 | 0 | 43 | 0 | 35 | 0 | 7 | 0 | 3 | 0 | 0 | MCAP_0329 | 1 TMB | Peptidase S41 | |
| ABC01574.1 | 8 | 161 | 9 | 209 | 0 | 6 | 0 | 0 | 0 | 0 | MCAP_0861 | 1 TMB | DUF342 | |
| ABC01698.1 | 4 | 106 | 10 | 80 | 30 | 43 | 19 | 18 | 17 | 5 | MCAP_0514 | lpp? | Transglutaminase-like superfamily | |
| ABC01774.1 | 0 | 69 | 3 | 57 | 7 | 8 | 5 | 3 | 3 | 0 | MCAP_0349 | 1 TMB | IgG-blocking virulence domain | |
| ABC01864.1 | 0 | 16 | 0 | 11 | 4 | 4 | 1 | 0 | 0 | 0 | MCAP_0345 | 1 TMB | IgG-blocking virulence domain | |
| Protein exosecretion reduced or abolished | ABC01302.1 | 34 | 187 | 20 | 118 | 81 | 36 | 109 | 29 | 172 | 6 | MCAP_0115 | 1 TMB | RecF/RecN/SMC N-terminal domain |
| ABC01413.1 | 15 | 75 | 16 | 65 | 32 | 44 | 34 | 17 | 32 | 3 | MCAP_0720 | lpp | lppQ | |
| ABC01444.1 | 12 | 59 | 13 | 38 | 22 | 11 | 25 | 2 | 20 | 0 | MCAP_0348 | lpp | Peptidase | |
| ABC01466.1 | 24 | 156 | 68 | 214 | 54 | 28 | 63 | 29 | 76 | 20 | MCAP_0240 | 2 TMB | Peptidase S41 | |
| ABC01836.1 | 3 | 26 | 5 | 18 | 23 | 0 | 27 | 0 | 16 | 0 | MCAP_0019 | 1 TMB | Secreted thousand residue frequently tandem | |
| Gene translation and protein exosecretion unmodified | ABC01469.1 | 0 | 23 | 0 | 19 | 1 | 22 | 7 | 39 | 4 | 17 | MCAP_0607 | lpp | Transglutaminase-like superfamily |
| ABC01669.1 | 0 | 13 | 0 | 15 | 0 | 28 | 0 | 11 | 0 | 3 | MCAP_0399 | 2 TMB | Hypothetical protein | |
| ABC01832.1 | 0 | 16 | 1 | 22 | 3 | 26 | 3 | 11 | 1 | 2 | MCAP_0401 | 2 TMB | Hypothetical protein | |
a
DUF, domain of unknown function; lpp, lipoprotein; P, pellet; S, supernatant; TMB, transmembrane region.
b
This protein is predicted to be 273 aa long in the Ck genome. However, orthologous genes in M. capricolum subsp. capricolum genomes are longer and contain one TMB.