Table 2.
Experimental runs for Box-Behnken design.
| Run | Coded | Actual | Yield (mg/mL) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| A | B | C | Temperature (°C) | Time (h) | IPTG (mM) | MICA | anti-MICA scFv | IL-23p19 | |
| 1 | −1 | −1 | 0 | 25 | 3 | 0.55/0.6 | 0.068 | 0.11 | 1.8 |
| 2 | 1 | −1 | 0 | 37 | 3 | 0.55/0.6 | 0.285 | 0.094 | 5.1 |
| 3 | −1 | 1 | 0 | 25 | 6/5 | 0.55/0.6 | 0.249 | 0.177 | 1 |
| 4 | 1 | 1 | 0 | 37 | 6/4 | 0.55/0.6 | 0.633 | 0.067 | 2.8 |
| 5 | −1 | 0 | −1 | 25 | 4.5/4 | 0.1/0.2 | 0.311 | 0.077 | 2.2 |
| 6 | 1 | 0 | −1 | 37 | 4.5/4 | 0.1/0.2 | 0.563 | 0.085 | 4.2 |
| 7 | −1 | 0 | 1 | 25 | 4.5/4 | 1 | 0.103 | 0.133 | 2 |
| 8 | 1 | 0 | 1 | 37 | 4.5/4 | 1 | 0.415 | 0.068 | 3.9 |
| 9 | 0 | −1 | −1 | 31 | 3 | 0.1/0.2 | 0.249 | 0.083 | 2.1 |
| 10 | 0 | 1 | −1 | 31 | 6/5 | 0.1/0.2 | 0.426 | 0.133 | 1 |
| 11 | 0 | −1 | 1 | 31 | 3 | 1 | 0.248 | 0.123 | 2.8 |
| 12 | 0 | 1 | 1 | 31 | 6/5 | 1 | 0.237 | 0.147 | 1 |
| 13 | 0 | 0 | 0 | 31 | 4.5/4 | 0.55/0.6 | 0.221 | 0.154 | 2.5 |
| 14 | 0 | 0 | 0 | 31 | 4.5/4 | 0.55/0.6 | 0.339 | 0.164 | 2.7 |
| 15 | 0 | 0 | 0 | 31 | 4.5/4 | 0.55/0.6 | 0.317 | 0.220 | 2.3 |
Coded and actual variables are shown. For time and IPTG, information shown is for MICA, anti-MICA scFv (left) and IL-23p19 (right).