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. 2019 Nov 14;9:16819. doi: 10.1038/s41598-019-53418-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OGD-PBMCs administration promotes both angiogenesis in the border area between the ischemic core and the penumbra and axonal outgrowth in the ischemic penumbra at 28 days after cerebral ischemia. (A) Representative figures and bar graphs representing the immunoreactivity for cluster of differentiation 31 (CD31) volume, expressed as μm³, in the ischemic core and penumbra from the cerebral cortices of the sham-operated group, and administration of the normoxic PBMCs group, 1 × 10⁵ OGD-PBMC, or 5 × 10⁵ OGD-PBMC groups at 28 days after cerebral ischemia. The CD31 (green)/MAP2 (red)/DAPI (blue) triple labelling in the ischemic cortices at 28 days after cerebral ischemia, as examined by confocal microscopy. (B) Representative figures and bar graphs representing immunoreactivity for SMI31-positive volumes, expressed as μm³, in the ischemic penumbra from the cerebral cortices of the administration of the normoxic PBMCs, 1 × 10⁵ OGD-PBMCs, or 5 × 10⁵ OGD-PBMCs groups. SMI31 (green)/DAPI (blue) double labelling in the ischemic cortices at 28 days after cerebral ischemia, as examined by confocal microscopy (N = 28). The bar graph represents the signal volume intensities of CD31 and SMI31 of brain samples by one-way ANOVA. Moreover, a secondary-only antibody control confirmed its specificity. Scale bars, 20 μm. **P < 0.01.