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. Author manuscript; available in PMC: 2020 Dec 31.

Published in final edited form as: Biochemistry. 2019 May 15;58(52):5305–5319. doi: 10.1021/acs.biochem.9b00292

Table 2.

Multiple Summed Exponential Fits for the Time Course of S5HH Single Turnover Reactions

	453 nm		700 nm
Salicylate	1/τ, s⁻¹	Amplitude (abs) [%]^a	1/τ, s⁻¹	Amplitude (abs)^a
1/τ₁	72 ± 5	−0.042 ± 0.0035 [63]	20.9 ± 1.1	−0.044 ± 0.0035
1/τ₂	3.5 ± 0.6	−0.006 ± 0.0005 [9.3]	0.48 ± 0.03	−0.002 ± 0.0004
1/τ₃	0.11 ± 0.01	−0.007 ± 0.0004 [11.0]	0.0014 ± 0.0002	+0.041 ± 0.0035
1/τ₄	0.003 ± 0.0005	−0.011 ± 0.0005 [16.5]
Benzoate
1/τ₁	4.1 ± 0.2	−0.035 ± 0.003 [58]	3.07 ± 0.25	−0.032 ± 0.003
1/τ₂	0.47 ± 0.05	−0.012 ± 0.001 [19]	0.32 ± 0.03	−0.005 ± 0.0005
1/τ₃	0.055 ± 0.006	−0.008 ± 0.001 [12.8]	0.002 ± 0.0002	+0.026 ± 0.002
1/τ₄	0.005 ± 0.0005	−0.006 ± 0.0005 [9.9]
No substrate
1/τ₁	0.144 ± 0.012	−0.030 ± 0.002	Not observed	Not observed
1/τ₂	0.065 ± 0.005	−0.019 ± 0.001	Not observed	Not observed

^a

A negative amplitude appears as an increase in absorbance during the time course