Extended Data Fig. 10. Generation of Unc93b1^S282A knock-in mice and B-cell stimulation with LPS.

(a) CRISPR/Cas-9 strategy to generate Unc93b1^S282A knock-in mice. Blue line indicates the guide sequence. Red bases indicated the edited codon. A representative sequencing trace of genomic DNA from an edited founder mouse is shown. (b) B cell proliferation in CSFE-labeled splenocyte cultures of the indicated mouse genotypes after stimulation for 3 days with increasing doses of LPS. The proliferation index is defined as geometric fluorescent intensity (gMFI) CSFE^Unstim: gMFI CFSE^Sample. Each curve shows the dose response of cells from three mice. Data are mean ± s.d. p values determined by two-way ANOVA followed by a Sidak’s posttest, ns = not significant. (c) Gating strategy for B cell stimulation assay.