Skip to main content
. Author manuscript; available in PMC: 2020 Mar 23.
Published in final edited form as: Nature. 2019 Sep 23;575(7782):366–370. doi: 10.1038/s41586-019-1612-6

Extended Data Fig. 4: Mass spectrometry analysis of Unc93b1 complexes.

Extended Data Fig. 4:

(a) A small fraction of Unc93b1 resides in endosomes compared to the endoplasmic reticulum (ER). Subcellular fractionation of TLR7-HA, Unc93b1-Flag expressing RAW macrophages was performed by density-gradient centrifugation. The distribution of Calnexin (ER), Lamp1 (late endosomes and lysosomes), Unc93b1-Flag, and TLR7-HA across fractions was measured by immunoblot. Representative of three independent experiments. (b) Workflow for isolation of phagosomes from RAW macrophages and purification of Unc93b1-Flag complexes from phagosome lysates.