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. 2019 Jun 26;38(4):159–163. doi: 10.12938/bmfh.18-029

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©2019 BMFH Press

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 1. — Quantitative PCR analysis of butyryl-CoA:acetate CoA-transferase (but) and butyrate kinase (buk) genes in feces and in the KUHIMM. (A, D) but in Roseburia sp./E. rectale, but (RosEub) (B, E) but in F. prausnitzii, but (F prsn), and (C,F) buk in C. butyricum, C. acetobutylicum, and C. perfringens. The percentages were calculated by quantitative PCR analyses (= 100 × [but or buk copy numbers]/[16S rRNA gene copy numbers]). Experiments were performed using DNA samples from (A–C) fecal samples and (D–F) KUHIMM fermentation cultures. * indicates significant difference, *p<0.05.