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. 2019 Jun 26;38(4):159–163. doi: 10.12938/bmfh.18-029

Table 1. Primers used in this study are illustrated.

Primer Name
(Forward)		 Base sequence Primer Name
(Reverse)		 Base sequence Genomic DNA s for standard curvesa Reference
G_buk_F tgctgtWgttggWagaggYgga G_buk_R gcaacIgcYttttgatttaatgcatgg Clostridium perfringens JCM 1290T [16]
G_Fprsn_F gacaagggccgtcaggtcta G_Fprsn_R ggacaggcagatRaagctcttgc Faecalibacterium prausnitzii JCM31915
G_RosEub_F tcaaatcMggIgactgggtWga G_Ros_R G_Eub_R tcgataccggacatatgccaKgag tcataaccgcccatatgccatgag Roseburia intestinalis JCM17583T
1132F atggYtgtcgtcagctcgtg 1108R Gggttgcgctcgttgc Faecalibacterium prausnitzii JCM31915

G_buk_F/R – buk genes of Clostridium acetobutylicum, C. butyricum, and C. perfringens; G_Fprsn – but gene of Faecalibacterium prausnitzii; G_RosEub, G_Ros_R, G_Eub_R – but genes of Eubacterium rectale and Roseburia sp.; 1132F, 1108R – universal primers for 16S.

a 16S rRNA gene copy numbers: 10 for Clostridium perfringens [29], 9 for Faecalibacterium prausnitzii [30], and 1 for Roseburia intestinalis (GenBank: FP929049.1).