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. 2019 Nov 14;9:16843. doi: 10.1038/s41598-019-53317-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Caskin1 does not influence cLTP-induced morphological changes in hippocampal neuronal cultures. (A) Representative images of CD1 and Caskin dKO neurons under control conditions or 5 hours after a 3-min induction by glycine treatment (cLTP). Arrows indicate mushroom spines of EGFP expressing dendritic branches, stained with anti-Shank2. Bars: 1 µm. (B,C) Mean relative protrusion density on the tertiary branches of EGFP-expressing CD1 (B) or Caskin dKO (C) hippocampal neurons with or without cLTP treatment. Protrusion types are categorized according to their morphology (stubby, filamentous or mushroom) and Shank2 positivity. (C) Caskin dKO neurons were transfected with either EGFP only or EGFP and V5-tagged Caskin1 constructs, 24 hours before the cLTP treatment. Data were obtained from 3–4 independent cultures. All data are displayed as mean ± SEM. Asterisks represent significance compared to control cultures and the $ symbol indicates significant differences between protrusion categories. *p < 0.05; **p < 0.01, ^$p < 0.05. The number of analysed protrusions is indicated above the columns.