Figure 1. Deep mutational scanning of PilE.

1. Representation of the overall work flow used in the study with the three selection schemes of pilE mutants and subsequent NGS‐based analysis.
2. Volcano plots of piliation, aggregation, and adhesion mutation scores for all pilE mutations. Stop and synonymous mutations are highlighted in blue and orange, respectively.
3. Adhesion and aggregation mutation scores relative to piliation. The correlation appears as a red dotted line.