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. 2019 Nov 7;10:2613. doi: 10.3389/fimmu.2019.02613

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Copyright © 2019 Nalian, Umstead, Yang, Silveyra, Thomas, Floros, McCormack and Chroneos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of domain deletion and site-directed mutagenesis on SP-A binding to SP-R210. The recombinant SP-A binding domain of SP-R210 was coated onto microtiter well plates, and incubated with increasing concentration of indicated recombinant rat SP-A proteins (A–D), or native human or rat SP-A proteins (C,D). Bound protein was detected using an HRP-conjugated SP-A antibody. Binding data were fitted by non-linear regression to generate binding isotherms (A,C) or transformed into double reciprocal plots to evaluate SP-A binding behavior (B,D). Assays were performed in duplicate or triplicate and data pooled from 2 independent experiments. Data shown are Means ± SE.