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. 2019 Nov 8;7:271. doi: 10.3389/fcell.2019.00271

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Copyright © 2019 Cui, Wang, Liu, Liu, Zhang, Li, Zhu, Pan, Zhang and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of ZFAS1 suppresses HB cell proliferation, migration and invasion. (A) qRT-PCR analysis of ZFAS1 expression in normal liver cell lines (L02 and Chang Liver) and HB cell lines (HepG2 and HuH-6). (B) qRT-PCR analysis of ZFAS1 expression in HepG2 or HuH-6 cells transfected with siSCR or si-ZFAS1. Cell proliferation capacity was analyzed by CCK-8 assay (C,D), EDU staining assay (E) and colony formation assay (F). (G) The invasion capability of HepG2 or HuH-6 cells transfected with NC or si-ZFAS1 was analyzed by transwell assay. Transwell assays were repeated for three times and relative quantification analysis was based on grayscale values. (H) The migration capability of HepG2 or HuH-6 cells transfected with NC or si-ZFAS1 was analyzed by wound-healing assay at indicated time points. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.