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. 2019 Nov 8;7:271. doi: 10.3389/fcell.2019.00271

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Copyright © 2019 Cui, Wang, Liu, Liu, Zhang, Li, Zhu, Pan, Zhang and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ZFAS1 negatively regulates miR-193a-3p expression. (A) Graphical representation of the potential binding sites between ZFAS1 and miR-193a-3p. (B) The relative luciferase activity of pZFAS1-miR-193a-3p-wt and pZFAS1-miR-193a-3p-mut was tested. (C,D) RIP assay was performed to determine the association between ZFAS1 and miR-193a-3p in HepG2 cells. (E) miR-193a-3p expression level in HepG2 cells transfected with ZFAS1 or si-ZFAS1 was shown. (F) miR-193a-3p expression level in HuH-6 cells transfected with ZFAS1 or si-ZFAS1 was shown. (G) miR-193a-3p expression in HB tissues were quantified by qRT- PCR analysis. (H) The negative correlation between ZFAS1 and miR-193a-3p was displayed by Pearson’s correlation curve. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.