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. 2019 Sep 19;179(1):120–131.e13. doi: 10.1016/j.cell.2019.08.034

Figure 2.

Figure 2

The Dimerization Domain Is Not Necessary for the Closure of Talin

(A) Negative-stain EM image of talin-ΔDD lacking the dimerization domain at 75 mM KCl, and 2D averages of the particles (right), showing a morphology similar to that of talin-FL.

(B) Size-exclusion chromatography coupled with multi-angle light-scattering profile of talin-FL and talin-ΔDD at 75 mM KCl, showing molecular weights corresponding to monomers. LS, light scattering, normalized; UV, absorbance.

(C) Centrifugation profiles of talin-ΔDD and talin-FL using the GraFix method. Talin-FL has an extra band in the fraction at higher sucrose density (open arrowhead). Black triangles depict the gradient of sucrose and glutaraldehyde concentrations.