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. 2019 Sep 19;179(1):120–131.e13. doi: 10.1016/j.cell.2019.08.034

Figure 6.

Figure 6

Talin-F-Actin Binding Assay

(A) Scheme of actin-binding domains ABS2 and ABS3 in the context of the autoinhibited talin structure.

(B) Table summarizing tested constructs for F-actin binding and vinculin accessibility of talin in compact conformation (75 mM KCl).

(C) Representative SDS-PAGE analysis of the F-actin co-sedimentation assay with various talin constructs at 75 mM salt. The dotted line indicates the elimination of an irrelevant lane from the gel.

(D) Quantification of the co-sedimented talin fragments. Data are represented as mean ± SD. N-R12, 0.68 ± 0.08; N-R11, 1.3 ± 0.2; N-R11 + V-head, 1.1 ± 0.3; R1-C, 1.1 ± 0.2; and R1-C + V-head, 1.3 ± 0.4. Differences between talin-FL and N-R12 as well as N-R11 are statistically significant (marked with ∗).

See also Figure S7.