Figure S2.

Quality Control of Cryo-EM Data Processing, Related to Figure 1

A: Local resolution map of the talin-FL structure, showing a resolution range between 5.5 Å and 7.9 Å. B: The Fourier shell correlation (FSC) curve of the talin-FL structure showing a global resolution of 6.2 Å. The additional line indicates a FSC of 0.143 C: Angular distributions of the particle orientations that were incorporated into the final 3D reconstruction. D: Cryo-EM images of talin-FL in the presence of 200 mM KCl and 500 mM KCl, showing aggregations presumably due to the talin opening at 500 mM KCl. 2D classifications of talin-FL at 200 mM KCl reveal less robust averages indicating higher structural heterogeneity.