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CCK-8 assay showed the cytotoxic effect of JSD on inhibition rate of cell proliferation in SW480, SW620 and HCT-8 cells. Cells were treated with JSD at the concentrations of 0, 1mg/ml, 2mg/ml, 4mg/ml, 8mg/ml, 16mg/ml, 32mg/ml, or 64mg/ml. Inhibitory effects were increased with the increase of JSD concentration. The half inhibition concentration (IC50) of JSD was 12.14mg/ml in SW480 cells, 12.7mg/ml in SW620 cells and 10.01mg/ml in SW620 cells; n=9.
