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. 2019 Oct 20;10(25):6439–6456. doi: 10.7150/jca.32873

Figure 3.

Figure 3

JSD-induced reversion on EMT progression of colon cancer cells in vitro. The experimental groups consisted of control, EMT, and JSD treated. The EMT and JSD groups were treated with 50 ng/mL EGF for 48 h. Next, the JSD group was treated with 6 mg/mL JSD for 48 h. (A) Western blot assays in SW480 cells. (B) and (C) Relative expression of indicated proteins in SW480 cells. (D) Western blot assays in SW620 cells. (E) and (F) Relative expression of indicated proteins in SW620 cells. (H) Western blot assays in HCT-8 cells. (I) and (J) Relative expression of indicated proteins in HCT-8 cells. Compared to the control group, the expression of E-cad was decreased, while N-cad, Vimentin and EMT related transcription factors Snail, Slug, Twist were increased in the EMT group (all, P<0.05). Additionally, the p-AKT1, AKT1 and p-GSK-3β levels in the EMT group were higher than those in the control group (all, P<0.05). On the contrary, E-cad was significantly upregulated in the JSD group in comparison with the control and EMT groups (all, P<0.05), while N-cad, Vimentin, p-AKT1, AKT1, p-GSK-3β, Snail, Slug and Twist were downregulated (all, P<0.05). Compared with NC-control group, *P<0.05, **P<0.01, ***P<0.001; compared with EMT group, ●●●P<0.001. n=3.