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. 2019 Oct 20;10(25):6439–6456. doi: 10.7150/jca.32873

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AKT1 was successfully overexpressed in SW480 and SW620 cells. (A): Real-time quantitative PCR (qPCR) showed that mRNA expression of AKT1 in SW480 and SW620 cells transfected with oe-AKT1 was significantly increased compared with that of the two cell lines transfected with NC. (B-C): Western blotting showed that the protein expression of AKT1 in SW480 and SW620 cells transfected with oe-AKT1 was significantly increased compared with that of the two cell lines transfected with NC. Compared with NC group, **P<0.01, ***P<0.001; n=3.