Figure 4.

Liensinine increases ROS levels and inhibits the PI3K/AKT pathway in gastric cancer cells. A) BGC823 and SGC7901 cells were treated with various concentrations of liensinine for 48 h, followed by incubation with the fluorescent probe DCFH-DA (10μM) for 30 min, ROS generation was detected using a microplate reader. B) ROS generation were determined in the absence or presence of 10 mM NAC for 2h. C) The protein levels of P-PI3K and P-AKT were examined by western blotting analysis.