Figure 4.

a) Full fluorescence titration of a 5 μm AP 1 and 50 μm CB[8] mixture upon addition of insulin (0–147 μm) (λ _ex=340 nm) in 10 mm HEPES buffer, pH 7.4. b) Initial stage of titration upon insulin addition (0–50 μm) and c) final stage of titration upon insulin addition (50–147 μm). d) Ratiometric fluorescence response (I ₃₈₆/I ₅₀₄) of insulin (0–147 μm) addition to the 5 μm AP 1 and 50 μm CB[8] chemosensing ensemble.