Figure 2.

Yield and size distribution of 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine (POPC) GUVs produced by varying the inner solution density and at multiple centrifugation speeds. a) Yields obtained for each concentration of inner sucrose solution tested at various centrifugation speeds. See the Experimental Section for yield calculation details. b) Size of the GUVs at different sugar concentrations and applied centrifugation speeds (n=3). c) Confocal images of GUVs containing 600 mm sucrose solution at various centrifugation speeds. Note that at 400 g bursting occurs. Here, the inner volume was 5 μL and the incubation time for the interface formation was 30 min with a lipid concentration of 400 μm. Scale bars: 50 μm.